Skip to main content
. 2020 Jul 9;39(16):e104730. doi: 10.15252/embj.2020104730

Figure 1. CAR forms microclusters that are signaling‐active.

Figure 1

  1. Domain structure of CAR.
  2. Schematic of the system for stimulating CAR T cells and imaging CAR dynamics. Supported lipid bilayers (SLBs) were coated with the extracellular domain of CD19 and an integrin ligand ICAM‐1.
  3. TIRF microscopy revealed the time courses of the CAR‐GFP microcluster formation as CAR T cells spread on the CD19‐ and ICAM‐1‐coated SLBs.
  4. Enrichment of phosphotyrosine (pY) at CAR microclusters. CAR T cells were fixed 20 min after being plated on the SLB, followed by staining with an anti‐pan phosphotyrosine antibody. No CD19 was included in the control experiment (only ICAM‐1). Scale bar: 2 μm.