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. 2020 Jul 9;39(16):e104730. doi: 10.15252/embj.2020104730

Figure EV2. LAT‐independent CAR microcluster formation in J.LAT cells.

Figure EV2

  1. WT or LAT‐deficient (J.LAT) Jurkat cells expressing CAR were stimulated on supported lipid bilayers coated with OKT3 (anti‐TCR antibody) or CD19 (CAR antigen) supplemented with ICAM‐1, respectively. TIRF microscopy revealed clustering of Ax647‐labeled streptavidin–biotin‐OKT3 or CD19, which serves as a probe for TCR or CAR, respectively. Scale bar, 2 μm.
  2. Quantification of clustering as normalized variance. n = 200 cells for each condition. Shown are the mean ± SD. Statistical test: unpaired two‐tailed t‐test. ****P < 0.0001, n.s., P = 0.4208.