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Supported lipid bilayers were coated with OKT3 (left) or CD19 (right) supplemented with ICAM‐1. Wild‐type or LAT‐deficient (Jcam2.5) T cells expressing ZAP70‐mCherry and CAR‐GFP were plated on the SLB and imaged by TIRF microscopy. Scale bar: 2 μm.
