Figure EV5. 1^st‐generation CAR forms microclusters and immunological synapses in LAT‐knockout primary T cells.

1. WT or LAT‐knockout (lat‐) primary T cells expressing the 1^st‐generation CAR were stimulated on SLBs coated with biotin‐CD19 and his‐ICAM‐1. TIRF microscopy revealed clustering of Ax647‐labeled streptavidin–biotin‐CD19 and CAR. Scale bar, 5 μm.
2. Quantification of clustering level of Ax647‐labeled streptavidin–biotin‐CD19. Central band: mean; box: quartiles; whisker: rest of the distribution. n = 123 cells for WT, 106 cells for lat‐. Clustering is quantified as normalized variance. n.s. P = 0.67. Statistical test: unpaired two‐tailed t‐test.