Skip to main content
. 2020 Jul 13;39(16):e105332. doi: 10.15252/embj.2020105332

Figure EV3. Effects of dynamin inactivation on membrane morphology.

Figure EV3

  • A, B
    Older shibire ts terminal cell that had already formed a long branch and tube before dynamin inactivation, fixed 15 min after inactivation. (A) Reconstruction of the entire cell. (A′, A″, B, B′) Higher magnification details of the cell, tomograms and reconstructions.
  • C
    shibire ts terminal cell expressing PH::mCherry. Red arrowheads point to puncta of fluorescent material at the tube membrane.
  • D–F
    TEM tomograms and 3D reconstructions of older shibire ts terminal cells similar to (A), but after 1 h (D–E) and 2 h (F) at restrictive temperature. Box in (D) is magnified in (E). (F″) The position at which the sheet between apical and basal is connected to the basal membrane is traced in red on the outside view of the basal membrane. The cells shown in (D–F) were found and acquired without the CLEM approach.
Data information: Scale bars: 500 nm (A, D, F), 200 nm (B, E), 5 μm (C).