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. 2020 Jul 13;39(16):e105332. doi: 10.15252/embj.2020105332

Figure 4. The role of endocytosis in terminal cell growth.

Figure 4

  • A–E
    Distribution of the plasma membrane reporter PH::GFP in control cells (A–A″) and in cells where dynamin activity had been blocked using a temperature‐sensitive allele of dynamin (shibire ts, B, E). In (B), it was inactivated at the onset of tube formation, whereas in (E), it was inactivated ˜15 min after the tube had begun to form. (C–D) Distribution of PH::GFP fluorescence intensity in control and shibire ts cells. Data from 1‐ to 2‐min interval time lapses were collected in windows of 20 min each (except for t = 0). Box plots represent median, interquartile range (IQR) and IQR*1.5 below and above the IQR. (C) Proportion of signal in the apical and in the basal membrane compartment over time in control cells (n = 4) and also in cells where dynamin was inactivated (n = 5), *P = 0.015, Mann–Whitney U‐test. (D) Total fluorescence intensity of PH::GFP over time in controls (n = 4) and in cells where dynamin was inactivated (n = 5), n.s.: P = 0.063, Mann–Whitney U‐test.
  • F, G
    Distribution of the luminal reporter Gasp::GFP in control cells (F) and in cells where dynamin was inactivated (G). Arrowheads point to protrusions sprouting from the lumen of the terminal cell.
  • H, I
    Distribution of the general plasma membrane marker CD4::mIFP in combination with the clathrin light chain fused to GFP (CLC::GFP, H) and with Dynamin::YFP (I).
Data information: Scale bars: 5 μm. Source data are available online for this figure.