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. 2020 Aug 17;16:294. doi: 10.1186/s12917-020-02518-w

Fig. 2.

Fig. 2

Effect of high density-induced stress on the IGF-1 system. a Representative Western blots of key genes of IGF-1 system, ERK and p4E-BP1, in phosphorylated and total forms, with H2B as the loading control. b Fold change of pERK/ERK ratio (c) Fold change of p4E-BP1/4E-BP1 ratio. Results are shown as the means ± SEM (n = 5 per group). LD, low stocking density; HD, high stocking density. Significant differences are indicated with a line (* = P < 0.05)