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. 2020 Aug 14;11:4084. doi: 10.1038/s41467-020-17915-w

Fig. 1. 2-HOBA attenuates atherosclerosis in hypercholesterolemic female Ldlr−/− mice.

Fig. 1

8-week-old Ldlr−/− female mice were pretreated with 1 g/L 2-HOBA or 1 g/L 4-HOBA (nonreactive analog) or vehicle (water) for 2 weeks and then the treatment was continued for 16 weeks during which the mice were fed a Western diet. Representative images (a) and quantitation (b) of Oil-Red-O stained proximal aorta root sections. Scale bar = 500 µm. n = 9 (vehicle), 10 (4-HOBA), or 9 (2-HOBA) biologically independent mice. Data are presented as mean ± SEM, One-way ANOVA with Bonferroni’s post hoc test, p values of 2-HOBA vs 4-HOBA and 2-HOBA vs vehicle are ***0.0009 and ***0.0004. Representative images (c) and quantitation (d) of Oil-Red-O stained open-pinned aortas. n = 10 biologically independent mice per group. Data are presented as mean ± SEM. One-way ANOVA with Bonferroni’s post hoc test, p values of 2-HOBA vs 4-HOBA and 2-HOBA vs vehicle are **0.0055 and **0.0033. e The plasma total cholesterol and triglyceride levels. n = 10 biologically independent mice per group. Data are presented as mean ± SEM. One-way ANOVA with Bonferroni’s post hoc test, p values of 2-HOBA vs 4-HOBA and 2-HOBA vs vehicle are >0.9999 for both cholesterol and triglyceride levels. Source data are provided as a source data file.