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. 2020 Aug 14;11:4084. doi: 10.1038/s41467-020-17915-w

Fig. 2. 2-HOBA decreases the MDA adduct content of proximal aortic atherosclerotic lesions in Ldlr−/− mice.

Fig. 2

a, b MDA was detected by immunofluorescence using anti-MDA primary antibody and fluorescent-labeled secondary antibody (red). Nuclei were counterstained with Hoechst (blue). Representative images (a) and quantitation (b) of MDA staining in proximal aortic root sections. Scale Bar = 50 µm. n = 6 biologically independent mice per group. Data are expressed as mean ± SEM. One-way ANOVA with Bonferroni’s post hoc test, p values of 2-HOBA vs 4-HOBA and 2-HOBA vs vehicle are **0.0017 and ***0.0008, respectively. c Aortic tissues were isolated from the Ldlr−/− mice and Dilysyl-MDA crosslinks were measured by LC/MS/MS. n = 9 biologically independent mice per group. Data are presented as mean ± SEM. Two-sided unpaired t test, p value of 2-HOBA vs 4-HOBA is *0.0262. d Aortic tissues were isolated from the Ldlr−/− mice and IsoLG-Lysyl was measured by LC/MS/MS. n = 9 (4-HOBA) or 8 (2-HOBA) biologically independent mice. Data are presented as mean ± SEM. Two-sided unpaired t test, p value of 2-HOBA vs 4-HOBA is *0.0151. Source data are provided as a source data file.