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. 2020 Aug 14;11:4084. doi: 10.1038/s41467-020-17915-w

Fig. 4. 2-HOBA prevents cell death and increases efferocytosis in atherosclerotic lesions of Ldlr−/− mice.

Fig. 4

a, c Representative images a and quantitation c of TUNEL-positive nuclei (red) of proximal aorta sections. Macrophages were detected by anti-macrophage primary antibody (green), and nuclei were counterstained with Hoechst (blue). Scale bar = 50 μm. n = 8 biologically independent mice per group. Data are presented as mean ± SEM. One-way ANOVA with Bonferroni’s post hoc test, p values of 2-HOBA vs 4-HOBA and 2-HOBA vs vehicle are *0.0134 and *0.0108. Higher magnification images of the TUNEL staining (b) were used to quantitate (d) efferocytosis of dead cells in aortic root sections. A representative image taken at a higher magnification shows macrophage-associated TUNEL stain (white arrows) and free dead cells (yellow arrows) that were not associated with macrophages (b). Scale bar = 50 μm. Efferocytosis was quantitated as the free vs macrophage-associated TUNEL-positive cells in the proximal aortic sections (d). n = 8 biologically independent mice per group. Data are presented as mean ± SEM. One-way ANOVA with Bonferroni’s post hoc test, p values of 2-HOBA vs 4-HOBA and 2-HOBA vs vehicle are *0.0162 and *0.0187. Source data are provided as a source data file.