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. 2020 Aug 14;11(8):624. doi: 10.1038/s41419-020-02849-4

Fig. 1. HLI injury induced damage to gastrocnemius tissues in a mouse HLI model.

Fig. 1

a Representative pictures of the foot were taken at days 3 and 7 after HLI injury. b The ischemic hindlimb was evaluated using ischemia scoring as described. (grade 0, no damage; grade 1, damaged claws; grade 2, damaged toe; grade 3, damaged to all toes; and grade 4, damaged foot). c The blood flow index was examined at the indicated times using ICG. The blood flow index represents the overall blood volume information with respect to time. d H&E staining was performed using cross-sectioned gastrocnemius muscle 7 days after injury. The red-dotted circle shows the necrotized region. Scale bar = 50 μm. e Gastrocnemius tissue was also stained with Masson, and the red-dotted circle shows fibrosis. Scale bar = 25 μm.