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. 2020 Aug 14;11(8):624. doi: 10.1038/s41419-020-02849-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Immunostaining of p62 was performed using quadriceps tissue from HLI mice. Nuclei were counterstained with hematoxylin. Scale bar = 25 μm. b–d, f Double immunofluorescence staining was performed using specific antibodies as indicated. p62 and CD31 (b); LC3 and CD31 (c); LC3 and dystrophin (d); and LC3 and LAMP2 (f). Nuclei were stained with Hoechst 33342. Scale bar = 20 μm. e, g The total LC3 signal and the overlapped LC3 signal with the indicated protein were quantified using Image J software and plotted. *p < 0.05, ***p < 0.001 versus control.