Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Aug 14;10:13838. doi: 10.1038/s41598-020-70788-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Bone metastasizing breast cancer cells acquire a mesenchymal phenotype. (A) Representative bright field images of in vitro cultured 4T1 and 4T-Bone cells, ×20 Magnification. Inset depicts an enlarged field. (B, C) qPCR analysis of the expression of EMT (B) and osteomimicry (C) related genes from 4T1 and 4T-Bone cell lines in vitro. Results presented are average of three biological repeats, normalized to 4T1 group. Paired t-test; *p < 0.05. Error bars represent SD of biological repeats. (D) Representative images of 4T1 and 4T-Bone breast cancer tissues showing (from left to right): H&E staining, scale bar-200 μm; immunofluorescence staining of Ecad (red); pan-cytokeratin (yellow); vimentin (pink); Ncad (green), nuclei were stained with DAPI (blue), scale bars-25 μm. (E–H) Quantification of IHC staining showed in (D), each dot represents a different field of view from multiple slides; n = 3 mice per group. Mean fluorescent intensity (MFI) was quantified using ImageJ and normalized to secondary antibody-only controls. Mann–Whitney test; *p < 0.05. (I) qRT-PCR analysis of EMT-related gene expression in primary 4T1 and 4T-Bone breast tumors. Results are presented as fold change of the average expression in 4T1 tumors. Each dot represents a different mouse. n = 6–9 mice, as indicated in graphs; Error bars represent SEM. *p < 0.05; Mann–Whitney test.