Fig. 2. UCHL1 deletion suppresses lipid uptake.

Macrophages were exposed to DMSO, oxLDL (50 μg/ml), LDN57444 (20 μM), and LDN57444 + oxLDL for 24 h. UCHL1 siRNA (50 nM) retreated for 24 h, then adding oxLDL (50 μg/ml) for additional 24 h in macrophages. Before cells were harvest for 6 h, Dil-oxLDL were used to incubate cells. The images were captured by confocal microscopy (a, b) and by flow cytometry (e, f). The showed images were from three experiments. c, d, g The quantitative analysis was presented. *p < 0.05, **p < 0.01, ***p < 0.001 versus oxLDL treatment group.