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. 2020 Aug 15;11(8):636. doi: 10.1038/s41419-020-02888-x

Fig. 3. Deletion of UCHL1 induces downregulation of scavenger receptor CD36 protein.

Fig. 3

a, b THP1 and RAW264.7 cells were treated with LDN57444 (5, 10, 20 μM) or UCHL1 siRNA (50 nM) for the indicated time. Western blot analysis for SR-A, CD36, SR-B, ABCA1, ABCG1, and Lox-1. c, d Peritoneal macrophages (pMΦ) were treated with UCHL1 siRNA or LDN57444 and cells lysates were subjected to western blot assay for CD36. e The treated cells were subjected to RT-PCR analysis for CD36. f Macrophages were exposed to cycloheximide (CHX) for the indicated times and LDN57444 or UCHL1 siRNA in combination with CHX for the same time. Western blot analysis for CD36 expression. h THP1 cells were exposed to MG132 for 6 h and then added LDN57444 treatment for 24 h. The protein expression of CD36 was tested. GAPDH was as the loading control. g, i The bands of CD36 proteins were calculated. *p < 0.05 versus LDN57444 treatment group.