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. 2020 Mar 9;27(9):2586–2604. doi: 10.1038/s41418-020-0522-3

Fig. 5. Angiotensin-(1–9) activates PKA signaling via miR-129-3p-dependent inhibition of PKIA.

Fig. 5

a Top ranking list of target genes obtained from TargetScan 7.2, filtered using http://pantherdb.org (reference proteome dataset), by biological process and biological regulation. b Schematic representation of the miR-129-3p and its seed region targeting the 3’UTR PKIA gene, displaying different prediction parameters in terms of conservation, mismatching, and predicted efficacy based on TargetScan 7.2. c Quantification of PKIA mRNA levels using RT-qPCR (n = 5), *p < 0.05. d pCREB, CREB, and β-tubulin levels were assessed by western blotting of cells treated with 100 μM angiotensin-(1–9) for the indicated times (n = 3). e, f Densitometric quantification of the western blots depicted in d. *p < 0.05 vs. 0 h.