Fig. 3. HSPA12A deficiency promoted Casnpase-11-mediated pyroptosis in both mouse livers in vivo and primary hepatocytes in vitro.

a Liver tissues were collected from mice 6 h after LPS or normal saline (NS) treatment. In another set of experiments, cultured primary hepatocytes were collected after LPS or NS incubation for 6 h. The following analysises were performed. Caspase-11 mRNA expression was evaluated using real-time PCR. Data are mean ± SD, *P < 0.05 and **P < 0.01 by two-way ANOVA followed by Tukey’s test. n = 6–10/group. Caspase-11 activation and GSDMD cleavage was examined in livers (b) and primary hepatocytes (c) using immunoblotting. Blots for GAPDH served as loading controls. Data are mean ± SD, *P < 0.05 and **P < 0.01 by two-way ANOVA followed by Tukey’s test. n = 6/group.