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. 2020 Apr 24;27(9):2651–2667. doi: 10.1038/s41418-020-0536-x

Fig. 5. HSPA12A deficiency suppressed AOAH expression in both mouse livers in vivo and primary hepatocytes in vitro.

Fig. 5

Liver tissues were collected from mice 6 h after LPS or normal saline (NS) treatment. In another set of experiments, cultured primary hepatocytes were collected after LPS or NS incubation for 6 h. The following analysises were performed. a Liver Aoah mRNA expression was evaluated using real-time PCR. Data are mean ± SD, *P < 0.05 and ** P < 0.01 by two-way ANOVA followed by Tukey’s test. n = 6/NS group and n = 11/LPS group. b Liver AOAH protein expression was evaluated using immunoblotting. Data are mean ± SD, **P < 0.01 by two-way ANOVA followed by Tukey’s test. n = 6 /group. c Primary hepatocyte Aoah mRNA expression was evaluated using real-time PCR. Data are mean ± SD, *P < 0.05 and **P < 0.01 by two-way ANOVA followed by Tukey’s test. n = 6/NS group and n = 5/LPS group. d Primary hepatocyte AOAH protein expression was evaluated using immunoblotting. Data are mean ± SD, *P < 0.05 and **P < 0.01 by two-way ANOVA followed by Tukey’s test. n = 6/group. e AOAH protein was immunostained in primary hepatocytes. Hoechst 33342 was used to counter stain nuclei. Data was expressed as fluorescence intensity/cell. Scale bar = 20 μm. Data are mean ± SD, **P < 0.01 by Student’s two-tailed unpaired t test. n = 3/group.