Fig. 2.

MiR-15a negatively regulated the expression of PD-L1. a, b The expression levels of miR-15a (a) and PD-L1 (b) were examined by RT-qPCR. c The protein level of PD-L1 was examined by western blot. DU145 and PC-3 were transfected with miR-15a mimics, miR-15a inhibitor, or the corresponding control miRNA (mimics NC or inhibitor NC). d Bioinformatic analysis using Starbase (http://starbase.sysu.edu.cn/index.php) revealed potential binding sites between miR-15a and PD-L1. e The relative luciferase activities were measured and compared between cells co-transfected with miR-15a mimics or miR-15a inhibitor. DU145 or PC-3 cells were transfected with luciferase reporter gene driven by either the wild type of PD-L1 (WT-PD-L1) or the mutated PD-L1 (MUT-PD-L1). *P < 0.05, **P < 0.01 and ***P < 0.001