Fig. 4.

MiR-15a regulated multiple malignant phenotypes of PC cells via directly targeting PD-L1. a–d Cell viability (a), apoptosis (b), migration (c), and invasion (d) of indicated PC cells were examined by MTT assay, Annexin V/PI staining followed by flow cytometry, wound healing assay, or Transwell assay, respectively. e The protein levels of Ras, p-ERK1/2, ERK1/2, Snail, E-cadherin, N-cadherin, MMP-9 from indicated PC cells were examined by western blot. DU145 and PC-3 cells were not transfected or transfected with miR-15a mimics, miR-15a mimics + pcDNA3.1-NC, or miR-15a mimics + pcDNA3.1-PD-L1, respectively. *P < 0.05, **P < 0.01 and ***P < 0.001