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. Author manuscript; available in PMC: 2021 Jul 13.
Published in final edited form as: Angew Chem Int Ed Engl. 2020 May 18;59(29):12178–12185. doi: 10.1002/anie.202005432

Figure 4. CAR-T activation mediated by cp-Fabs.

Figure 4.

The CAR-T was incubated with 20 nM of the indicated FOLR1-targeting cp-Fabs or the corresponding unprogrammed Fabs in the presence of IGROV-1 cells at an E:T ratio of 10:1 for 24 h. The percentage of activated T cells based on CD25 expression after incubation with cp-Fabs based on tagged (a) or WT (b) h38C2 Fabs was measured by flow cytometry. Cytokines released from the T cells in the presence of cp-Fabs based on tagged (c) or WT (d) h38C2 Fabs were measured by ELISA. Shown are mean ± SD values for independent triplicates. An unpaired two-tailed t-test was used to analyze significant differences (*, p < 0.05; **, p < 0.01; ***, p < 0.001).