Table 2. Characterization by MALDI-TOF, PDB Codes, BMRD ID, and Electrophysiology Effect of Current Inhibition of R-tam and Mutantsa.
| r-tam mutant | Oxidized form, Theoretical Mass | Experimental Mass | PDB ID Code | BMRB ID | Case | Effect of SK2 current inhibition | Effect of SK3 current inhibition |
|---|---|---|---|---|---|---|---|
| r-tam | 3459.1 | 3459.0 | 2LU9 | 18513 | ⧫⧫⧫ | ⧫⧫ | |
| ΔP30 | 3362.0 | 3361.8 | 6D3T | 30454 | 1 | ⧫⧫ | ⧫ |
| ΔP30/In32N | 3476.1 | 3476.1 | 6D8Q | 30460 | 1 | ⧫⧫ | ⧫ |
| Y31H | 3433.1 | 3433.4 | 6D8Y | 30465 | 1 | ⧫⧫ | ⧫ |
| In32N | 3573.2 | 3573.1 | 6D8H | 30459 | 1 | ⧫⧫ | ⧫ |
| K20E | 3460.1 | 3460.2 | 6D8U | 30464 | 2 | ⧫⧫⧫ | ⧫ |
| E25K | 3458.2 | 3457.9 | 6D8R | 30461 | 2 | ⧫ | ⧫⧫ |
| K27E | 3460.1 | 3460.0 | 6D8S | 30462 | 2 | ⧫ | ⧫⧫ |
| E25K/K27E | 3459.1 | 3458.9 | 6D8T | 30463 | 2 | ⧫⧫ | ⧫⧫⧫ |
| K20A | 3402.0 | 3402.1 | 6VNZ | 30719 | 3 | ⧫⧫⧫ | – |
| E25A | 3401.1 | 3401.0 | 6D9O | 30467 | 3 | ⧫ | ⧫⧫ |
| K27A | 3402.0 | 3402.3 | 6D9P | 30468 | 3 | ⧫ | ⧫⧫ |
| Y31A | 3367.0 | 3367.0 | 6D93 | 30466 | 3 | ⧫⧫ | ⧫ |
a
We also include the case described to include the mutant in our study. We evaluated all mutants base on high (⧫⧫⧫, > 85%), medium (⧫⧫, 84–40), low (⧫, < 39%), or absence activity (−) under electrophysiology activity on SK2 and SK3 channels.