. Author manuscript; available in PMC: 2021 May 14.

Published in final edited form as: J Med Chem. 2020 Apr 17;63(9):4528–4554. doi: 10.1021/acs.jmedchem.9b01573

Table 2.

Inhibition of rat and human nNOS compared to murine and human iNOS by selected compounds^a

	K_i (μM)				Selectivity
compd	rnNOS	hnNOS	miNOS	hiNOS	^rn/_mi	^hn/_hi
7	0.045	0.088	16.3	6.94	362	79
10	0.119	0.151	2.86	3.55	24	24
12	0.055	0.060	24.2	29.9	441	498
29	0.039	0.046	32.8	21.1	841	459
32	0.052	0.076	45.7	43.9	879	578
33	0.044	0.045	8.18	29.4	186	653
36	0.043	0.063	16.4	24.6	382	390
37	0.053	0.046	21.0	34.8	397	757

Compounds 7, 10, 12, 29, 32, 33, 36, and 37 were assayed for in vitro inhibition against purified human iNOS (hiNOS) using known literature methods, and K_i values were calculated directly from IC₅₀ values using the Cheng–Prusoff equation. IC₅₀ values are the average of at least two replicates from 6–9 data points; all experimental standard error values (for the LogIC₅₀) are less than ± 0.10. K_i values for isoforms: rat nNOS (rnNOS), human nNOS (hnNOS), and murine iNOS (miNOS) were included for comparison. Selectivity values are ratios of respective K_i values.