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. Author manuscript; available in PMC: 2021 Apr 15.
Published in final edited form as: J Proteomics. 2020 Jan 9;217:103645. doi: 10.1016/j.jprot.2020.103645

Figure 1.

Figure 1.

Test of different sample preparation methods. A. Comparison of the effect of Top 12 abundant protein depletion, direct in-solution digestion and S-Trap aid in column digestion method. B. Comparison of the fractionation method between high pH STAGE tip method and SDS-PAGE. Recovered protein (shown in Gene Protein Product, GPs) and Peptide Spectrum Matches (PSMs) numbers are representative results from triple repeat. C. Schematic illustration of established workflow in the serum profiling process. S-Trap aided trypsin digestion, high pH STAGE tip method for preparation of serum samples was used.