Table 3.
Experimental design and locations of reviewed studies that reported the prevalence of E. coli O157:H7 in Ethiopian dairy products.a
| Reference | Region | Town | Dates | Place of sampling | Study design | Sample size calculation | Detection method | Confirmation method |
|---|---|---|---|---|---|---|---|---|
| Mekuria and Beyene (2014) | Tigray | Mekele, Alamata and Adigrat | Nov-2012 to Jun-2013 | Collected from the udders of lactating animals and from the distribution points. | Cross-sectional | Sample size determined based on P = 50% | Streak on blood agar, transfer to BUG | Biolog identification system |
| Adugna et al. (2015) | Somali and Dire Dawa | Erer and Dire Dawa | Not stated | Collected from producers' households and markets | Not stated | Not stated | Spread plate in MacConkey agar | Sorbitol, arabinose and mannitol fermentation and indole test |
| Disassa et al. (2017) | Benish-angul-Gumuz | Asosa | Oct-2014 to Jul 2015 | Obtained from dairy farmers and milk vendors | Cross-sectional | Sample size determined based on P = 44.4% | Enrichment in EC broth, transfer to EMB agar, isolation on sorbitol McConkey agar | Agglutination test for O157 and H7 antigens |
| Abunna et al. (2018c) | Oromia | Asella | Nov-2016 to Apr-2017 | Collected from farmers, vendors, milk collection centres, markets and kiosks. | Cross-sectional | Not stated | Streak on McConkey agar, transfer to EMB, confirmation for E. coli then transfer to sorbitol McConkey | Agglutination test for O157 and H7 antigens |
| Bedasa et al. (2018) | Oromia | Bishoftu | Nov-2016 to Apr-2017 | Obtained from restaurants, open markets and supermarkets. | Cross-sectional | Not stated | ISO 16654:2001 | Subculture of confirmed E. coli isolates in sorbitol MacConkey agar and agglutination test for O157 and H7 antigens |
a
Abbreviations: P, prevalence; BUG, Biolog Universal Growth agar; EC, Escherichia coli broth; EMB, eosin methylene blue agar.