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. 2020 Apr 14;222(6):1037–1045. doi: 10.1093/infdis/jiaa184

Figure 5.

Figure 5.

Friend leukemia virus integration 1 (Fli-1) is a target of miR-145a and regulates NF-κB activation. A, Fli-1 3’ untranslated region (UTR) sequences containing miR-145a-5p–binding sites. B, Lung pericytes transfected with miR-145a-5p mimic (40 nmol/L) or negative control (NC; 40 nmol/L) for 48 hours. Expression levels of Fli-1 were determined (n = 3). †P < .05 (comparison with NC group). C, Lung pericytes were transfected with a control vector or constructs overexpressing Fli-1. The protein levels of the total and phosphorylated- NF-κB were detected with Western blot analysis (n = 3). *P < .05 (comparison with control group). D, The pericytes were transfected with Fli-1 small interfering RNA (siRNA) (50 nmol/L) or scrambled siRNA (50 nmol/L) and stimulated with lipopolysaccharide (LPS; 100 ng/mL) for 6 hours. The lysates were processed to measure total and phosphorylated p65 with Western blot analysis (n = 4) *P < .05 (comparison with control group); ‡P < .05 (comparison with LPS control group). E, Graphic depicting how miR-145a regulates lung vascular leakage by targeting NF-κB signaling via Fli-1.