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. Author manuscript; available in PMC: 2020 Aug 17.
Published in final edited form as: Cell Rep. 2020 Jul 28;32(4):107962. doi: 10.1016/j.celrep.2020.107962

Figure 2. The Transplanted iE3/3 and E4/4 Neurons Survive and Functionally Integrate into the Mouse Hippocampus at 7 MPT.

Figure 2.

(A–F) Representative immunohistochemical staining of transplanted iE3/3 and E4/4 neurons for HNa (A and D), NeuN (B and E), and human SYN (C and F). Scale bar, 25 μm.

(G and H) Representative immunohistochemical staining for mCherry+ (red) displayed distal projections emanating from the transplant core (G) (scale bar, 500 μm), with a magnified image of the inset (H) (scale bar, 50 μm).

(I) Whole-cell patch-clamp recordings in ex vivo slices of transplanted iE3/3 neurons (left) and E4/4 neurons (right) demonstrating capability to fire action potentials.

(J) Whole-cell patch clam recordings in ex vivo slices of transplanted iE3/3 neurons (top) and E4/4 neurons (bottom) demonstrating capability to receive sPSCs. Holding potential is −70 mV.