TABLE 2.

[³H]Prazosin saturation binding to HEK-α_1A-Adr, HEK-α_1B-Adr, and HEK-α_1D-Adr receptor subtypes: [³H]Prazosin K_D and B_max values

[³H]Prazosin was tested at concentrations ranging from 0.08 to 2.5 nM. Assays were conducted in duplicate. Experiments were conducted as described in the text.

Receptor	[3H]Prazosin Binding	KD (nM) ± S.E.M.
	Bmax (pmol/mg) ± S.E.M. (n)
α1A-Adr	6.39 ± 0.30 (3)	0.153 ± 0.052
α1B-Adr	7.7 ± 1.7 (3)	0.147 ± 0.018
α1D-Adr	1.12 ± 0.18 (3)	0.186 ± 0.003

n, number of independent experiments, each conducted with duplicate determinations.