Figure 3.

Pre-vaccination cyclophosphamide (CTX) increases the CD8⁺ T-cells/Tregs ratio and reduces proliferating Tregs in the periphery. Frequency of (A) total CD4⁺ T-cells, (B) total CD8⁺ T-cells and (C) CD4⁺ CD25⁺ FoxP3⁺ Tregs among the circulating lymphoid compartment of melanoma lung-metastatic mice. (D) Ratio of CD8⁺ T-cells/Tregs in peripheral blood samples taken 24 hours after adenovirus (Ad)-dopachrome tautomerase (DCT), either alone or after CTX preconditioning. Graphs display mean±SEM of two pooled experiments. Frequency of (E) proliferating 5-bromo-2'-deoxyuridine (BrdU)⁺ CD4⁺ CD25⁺ FoxP3⁺ Tregs and (F) ratio of CD8⁺ T cells/BrdU⁺ Tregs within the lymphoid compartment of blood samples collected 72 hours after treatment of melanoma lung-metastatic animals with Ad-DCT, administered either alone or after CTX preconditioning (untreated controls labeled “-"). Mean±SD displayed.