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. 2020 Aug 13;8(2):e000271. doi: 10.1136/jitc-2019-000271

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© Author(s) (or their employer(s)) 2020. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See http://creativecommons.org/licenses/by-nc/4.0/.

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Flow cytometry analysis of maturation stages of CD8+ T cells in different soft tissue sarcoma subtypes using the following definitions: naïve (CCR7+/CD45RA+, T_N), central memory (CCR7+/CD45RA−, T_CM), effector memory (CCR7−/CD45RA−, T_EM), and effector memory expressing CD45RA T cells (CCR7−/CD45RA+, T_EMRA). (A) Cumulative maturation stages in CD8+ T cells; (B) T_EM cells as percentage of CD8+ T cells. Included in analysis of (A) and (B): GIST (n=8), leiomyosarcoma (n=6), liposarcoma (n=10), myxofibrosarcoma (n=8), pleomorphic sarcoma (n=9), lipoma (n=3) and melanoma (n=4). *, represents p value of <0.05 according to the Mann-Whitney U test. GIST, gastrointestinal stromal tumor; T_CM, central memory T cell; T_EM, effector memory T cell; T_EMRA, effector memory expressing CD45RA T cells; T_N, naïve T cell.