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. Author manuscript; available in PMC: 2021 Aug 1.
Published in final edited form as: J Neurosci Res. 2020 May 19;98(8):1532–1548. doi: 10.1002/jnr.24637

Table 3.

Monoamine levels in the striatum.

Monoamines and Metabolites WT (n=18) MOR KO (n=21) Genotype X Time
Midday (n=8) Midnight (n=10) Age Time Midday (n=9) Midnight (n=12) Age Time
NA 7.20±2.06 3.86±1.04 F1,15=1.56 F1,15=20.16 6.88±1.62 4.20±1.42 F1,18=1.66 F1,18=16.29 F1,34=0.46
p=0.51 p<0.01** p=0.59 p<0.01** p=0.50
DOPAC 8.59±2.47 11.46±1.53 F1,15=7.26 F1,15=12.37 9.66±1.87 10.63±1.38 F1,18=4.93 F1,18=2.24 F1,34=3.45
p=0.10 p<0.01** p=0.43 p=0.33 p=0.07
DA 127.18±33.45 199.01±22.78 F1,15=1.52 F1,15=29.43 153.35±34.41 180.06±21.28 F1,18=2.92 F1,18=5.16 F1,34=6.72
p=0.43 p<0.01** p=0.38 p=0.10 p=0.01*
5-HIAA 4.53±0.83 5.24±1.18 F1,15=1.10 F1,15=1.91 4.98±0.93 4.66±0.87 F1,18=1.31 F1,18=0.64 F1,34=2.63
p=0.76 p=0.19 p=0.59 p=0.47 p=0.11
HVA 14.76±3.21 18.94±3.04 F1,15=2.42 F1,15=8.46 17.65±4.02 17.08±2.77 F1,18=4.91 F1,18=0.18 F1,34=5.86
p=0.39 p=0.02* p=0.22 p=0.68 p=0.02*
5-HT 22.39±3.94 19.62±1.77 F1,15=3.78 F1,15=4.57 21.31±2.63 18.60±2.11 F1,18=1.24 F1,18=6.76 F1,34=0.00
p=0.26 p=0.06 p=0.51 p=0.07 p=0.98
3-MT 9.09±2.38 11.37±1.77 F1,15=0.20 F1,15=5.01 10.45±2.09 11.42±2.46 F1,18=0.73 F1,18=0.87 F1,34=0.81
p=0.73 p=0.05* p=0.56 p=0.44 p=0.37
DOPAC/DA 0.07±0.01 0.06±0.01 F1,15=13.17 F1,15=16.11 0.06±0.01 0.06±0.01 F1,18=0.08 F1,18=1.63 F1,34=1.22
p=0.03* p<0.01** p=0.78 p=0.30 p=0.28
HVA/DA 0.12±0.01 0.10±0.01 F1,15=0.32 F1,15=15.82 0.12±0.01 0.10±0.01 F1,18=0.45 F1,18=16.33 F1,34=0.01
p=0.71 p<0.01** p=0.56 p<0.01** p=0.94
5-HIAA/5-HT 0.20±0.03 0.26±0.04 F1,15=0.85 F1,15=12.15 0.23±0.03 0.25±0.02 F1,18=0.57 F1,18=1.79 F1,34=4.51
p=0.58 p<0.01** p=0.56 p=0.31 p=0.04*
3-MT/DA 0.07±0.01 0.06±0.01 F1,15=0.65 F1,15=9.87 0.07±0.01 0.06±0.01 F1,18=1.05 F1,18=2.04 F1,34=1.72
p=0.59 p=0.01* p=0.50 p=0.31 p=0.20

The values of each parameter are presented in means ± SDs in ng/ul of tissue and p-values for interactions were calculated by mixed model ANOVA with age, genotype and time as factors. The p-values for age and time were calculated by mixed model ANOVA grouped by genotype. p-values have been adjusted for multiple comparisons using the Benjamini-Hochberg-Yekutieli false discovery rate [FDR (p < 0.05)].

*

p≤0.05;

**

p<0.01. NA, Noradrenaline; DOPAC, 3,4-Dihydroxyphenylacetic acid; DA, Dopamine; 5-HIAA, 5-Hydroxyindoleacetic acid; HVA, Homovanillic acid; 5-HT, Serotonin; 3-MT, 3-Methoxytyramine. WT, wild-type mice. MOR KO, mu opioid receptor knockout mice.