. Author manuscript; available in PMC: 2021 Aug 1.

Published in final edited form as: J Neurosci Res. 2020 May 19;98(8):1532–1548. doi: 10.1002/jnr.24637

Table 5.

Tail-flick test.

Groups	WT (1^st, n=14; 2^nd, n=5)			MOR KO (1^st, n=14; 2^nd, n=6)				Age X Genotype	Weight X Genotype	p for Age	p for Weight	p for Genotype
Groups	Estimate	SE	95% CI	Estimate	SE	95% CI	Z	Age X Genotype	Weight X Genotype	p for Age	p for Weight	p for Genotype
1^st cohort	0	0	0	−0.30	0.13	−0.55 and −0.05	−2.33	0.72	0.27	0.09	0.03^*	0.02^*
2^nd cohort Midnight	0	0	0	−0.30	0.18	−0.65 and 0.05	−1.70	0.11	0.06	0.53	0.79	0.09
2^nd cohort Midday	0	0	0	−0.42	0.11	−0.63 and −0.21	−3.95	0.19	0.23	0.75	0.25	<0.01^*
2^nd cohort Ropinirole	0	0	0	0.17	0.10	−0.02 and 0.36	1.78	0.98	0.22	0.22	0.40	0.08

The p-values for interactions were calculated by GENMOD with a gamma distribution with age, weight, and genotype as factors. The parameters and p-values for age, weight and genotype were calculated by GENMOD with a gamma distribution with the interaction removed.

p≤0.05;

^**

p<0.01. WT, wild-type mice. MOR KO, mu opioid receptor knockout mice. SE, standard error. 95% CI, 95% confidence interval.