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[Preprint]. 2020 Aug 11:rs.3.rs-55764. [Version 1] doi: 10.21203/rs.3.rs-55764/v1

Figure 4.

Figure 4

Lipid mediators attenuate IFNγ-induced ACE2 expression, senescence programming, and binding of Alexa 594-RBD in human corneal epithelial cells (HCEC). a, Among several cytokines tested only IFNγ and α, induces ACE2 expression in HCEC (6 hours after stimulation, analyzed by dd- PCR). b, Effect of lipid mediators on gene expression of Ace2, Cdkn2a, and Mmp1 of HCEC after adding IFNγ (100 ng/ml). ΔΔCT normalized fold change was used. p-values of statistical t-test analysis in comparison to vehicle group are shown. Mean and SD are shown as the lines. c, Alexa 594-RBD binding in HCEC. IFNγ (100 ng/ml) and lipid mediators (200 nM) were added to the HCEC for 12 h. Alexa 594- RBD (0.5 ng/well) was then added and images taken 24 h after. Fifteen images/condition analyzed. Representative images are shown (left side), and the Imaris based calculation was plotted (right-hand side). Data are presented as single image/each data point. The p-value of ANOVA-post hoc Dunnett's multiple comparisons test with vehicle as reference. Mean and SD are shown as the lines. d, SASP Secretome (β-Gal staining) of HCEC 24 h after IFN-γ challenge and +/− lipid mediators. Each point represents one image. The p-value of ANOVA-post hoc Dunnett's multiple comparisons test with vehicle as reference are shown. Mean and SD are shown as the lines. Representative images for each condition are in the right panel.