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[Preprint]. 2020 Aug 14:rs.3.rs-48659. [Version 1] doi: 10.21203/rs.3.rs-48659/v1

Figure 1: Schematic of TMPRSS2-HiBiT detection for high throughput screen.

Figure 1:

(A) The split nano-luciferase components LgBiT and HiBiT can interact to form a functional enzyme that generates luminescence. (B) Human TMPRSS2 cDNA was C-terminally tagged with a HiBiT sequence on a domain that is extracellular when TMPRSS2 is present in the plasma membrane. (C) The TMPRSS2-HiBiT construct was expressed in human airway cells where it exists in an intracellular pool and a plasma membrane-associated pool. (D) Non-lytic extracellular HiBiT detection results in LgBiT-HiBiT complementation solely with the pool of plasma membrane localized TMPRSS2. Following extracellular HiBiT detection, cells are lysed and the total TMPRSS2- HiBiT is then quantified.