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. 2020 Jul 1;48(14):8050–8062. doi: 10.1093/nar/gkaa543

Figure 2.

Figure 2.

miRNA quantification in oocytes and 3T3 cells. (A) qPCR-based quantification of miRNAs in 3T3 cells (∅ 18 μm) and rodent fully grown oocytes (∅ 80 μm). miRNA copy numbers per cell were estimated in three independent experiments using the standard curve in Supplementary Figure S1C. Error bars = standard deviation (SD). Logarithmic scale is used for y-axis. miR-322, miR-132 and miR-290 were not detected in 3T3 cells. (B) Northern blots for quantifying let-7a miRNA counts in mouse fully grown oocytes. Serially diluted let-7a synthetic oligonucleotide was used for standard calibration. (C) qPCR-based miRNA quantification in porcine oocytes (∅ 105 μm) and bovine oocytes (∅ 120 μm). miRNA copy numbers per cell were estimated in three independent experiments using the standard curve shown in Supplementary Figure S1C, except for hamster oocyte data, which came from a single oocyte collection. Error bars = SD. Logarithmic scale is used for y-axis.