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. 2020 Jul 1;48(14):8050–8062. doi: 10.1093/nar/gkaa543

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 5. — miRNAs do not accumulate during the oocyte growth and have higher turnover than mRNAs. (A) PCR-based quantification of miRNAs in 3T3 fibroblasts (∅ 18 μm), mouse growing oocyte (∅ 40 μm) and fully grown oocyte (∅ 80 μm). miRNA copy numbers per cell were estimated in three independent experiments using the standard curve in Supplementary Figure S1C. Error bars = standard deviation (SD). Logarithmic scale is used for y-axis. 3T3 cell and fully grown oocyte values are the same as in Figure 2A. (B) mRNA abundance in fully grown oocyte (∅ 80 μm) relative to mouse growing oocyte (∅ 40 μm). The mRNA abundance in the growing oocyte is set to one. mRNA abundance was estimated in three independent experiments per oocyte cDNA equivalent. Error bars = standard deviation (SD). (C) qPCR-based quantification of miRNA per oocyte equivalent during extended culture of fully grown oocytes in the presence of IBMX. miRNA abundance was estimated in two independent experiments. Error bars = standard deviation (SD). (D) mRNA abundance in fully grown oocytes during extended culture in the presence of IBMX relative to oocytes collected at 0 h, which is set to one. mRNA abundance was estimated per oocyte equivalent in two independent experiments. Error bars = standard deviation (SD).