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. 2020 Jul 1;48(14):7899–7913. doi: 10.1093/nar/gkaa560

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 7. — Expression of GFP with the Q103 leader sequence. Representative flow cytometry detected fluorescence profiles of Q103-GFP in Ax2 wild type and elp3⁻ (A), trm9⁻ (B), ctu1⁻ (C), elp3⁻/ctu1⁻ (D) and tQ^CUG− (E) mutant strains. Shown are expression profiles from the mutant strain (blue) versus Ax2 (olive, left), versus mutant strain +tQ^CUG overexpression (magenta, middle) and versus mutant strain +tQ^UUG overexpression (orange, right). Gray areas indicate the background signal from untransformed strains. Fluorescence is shown in arbitrary units. (F) Western blots against GFP with the Q103-leader expressed in the indicated mutant and recue strains, compared to Ax2 wild type. Coronin antibodies served as loading and transfer controls. Control: untransformed Ax2 wild type; M: PageRuler Plus Prestained Protein Ladder as size marker with relevant sizes shown to the left.