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. 2020 Jul 1;48(14):7899–7913. doi: 10.1093/nar/gkaa560

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 8. — Expression of GFP with the Q15 leader sequence. (A) Representative flow cytometry detected fluorescence profiles of Q15-GFP in Ax2 wild type compared to elp3⁻, trm9⁻, ctu1⁻ and tQ^CUG− mutant strains. Shown are expression profiles from the mutant strain (blue) vs. Ax2 (olive). Grey areas indicate the background signal from untransformed strains. Fluorescence is shown in arbitrary units. (B) Western blots against GFP with the Q15-leader expressed in the indicated mutant strains, compared to Ax2 wild type. Discoidin antibodies served as loading and transfer controls. M: PageRuler Plus Prestained Protein Ladder as size marker with relevant sizes shown to the left.