Figure 2.

dCas9 RNA oscillator ACF. (A) The 1-plasmid dCas9 RNA oscillator circuit (JK412) with (B) ACF calculated for individual cell traces. Selected representative traces are bolded. (C) The population-averaged ACF shows a slower decay, with the reporter signal YFP/RFP ACF decaying much slower than the ACF for segmentation marker RFP. (D) Some individual traces showed first correlation peaks across a wide range, with an average of 8.6 generations. (E–H) The same properties were seen for a version with sgRNAs transcriptionally flipped. Cells measured: (B) JK412, 2358; (F) JK413, 3037.