Figure 2.

Correction of existing misannotated transcripts of At4g17140 by Nanopore long reads. (A) Diagram showing the difference between three reference isoforms (1–3, black) and our novel transcripts/isoforms (A and B, gray). Six different regions (DRs) are highlighted with box, and the primers used to amplify this region are shown near the DRs. DR 1–3 are the difference between reference isoforms and both the novel isoform A and B; while the DR 3–6 are the difference between isoform B and isoform A. (B–D). A magnified view of DR 1–3 shown in ‘A’. Shown on the top are the primers used for PCR validation and the mapping result of Sanger sequencing. (E). RT-PCR results for the confirmation of DR 1–3. Only one major band for each PCR can be found, and the isoforms supported by the Sanger sequencing results for each PCR are shown under the band. (F). A magnified view of DR 4–6 shown in ‘A’. (G). RT-PCR results for the confirmation of difference 4–6. The weaker band for the expected product of isoform B are indicated by red arrows. B, floral buds; S, seedlings; maker, the DL2000 DNA ladder. DR, different region.