Figure 4.

Unexpected fusion transcripts identified with Nanopore reads are confirmed with RT-PCR. (A) Multiple fusion transcripts derived from two discrete existing loci; (B) a single exon transcript covering four gene locus; (C) a transcript covering two distal multi-exon genes together with seven single exon genes; (D) fusion transcripts covering two overlapped genes with the same splicing patterns, and there is no splicing in the overlapped regions of the two covered genes; (E) antisense transcripts covering two adjacent genes; (F) transcripts with different splicing pattern covering parts of two proximal gene loci. Existing exon and intron are depicted as red thin and thin bar respectively, exon and intron derived from Nanopore reads are depicted as blue thin line and thin bar respectively. The position of forward (F) and reverse (R) position are shown in the diagram. The RT-PCR confirmation results of the fusion transcripts are shown in the right of each panel. B, floral buds; S, seedlings; Maker, the 1 kb DNA ruler.