Figure 5.

Non-annotated DNA sequence confirmed in the second intron of At2g40980. (A) The IGV track view of reads mapping to gene At2g40980. The unannotated sequences fall in the second intron as shown along the red line. (B) PCR confirmation with the primers flanking the unannotated regions, the red line indicates the expected size according to TAIR10 genome. (C) The IGV track view of reads with the corrected Arabidopsis genome. Note: The expected size in TAIR10 is 176 bp, while with the non-annotated DNA fragment, the expected size should be 278 bp.