Table 1.
Summary of Nanopore native RNA read statistics
Stage | Seedling1 | Seedling2 | Buds1 | Buds2 |
---|---|---|---|---|
Read numbera | 707 220 | 736 859 | 982 453 | 780 408 |
Average length | 1003 | 983 | 1000 | 924 |
Median length | 851 | 828 | 820 | 764 |
N50 length | 1188 | 1143 | 1214 | 1141 |
Max read length | 24 734 | 20 411 | 57 484 | 24 080 |
Average read quality | 12 | 12 | 12 | 12 |
Alignment % identityb | 93% | 93% | 92% | 92% |
aThe Read number is the raw read number, excluding the ENO2 internal control.
bReference of cDNA and genome were fetched from Araport11, containing 48 359 protein coding isoforms in total.