Figure 7.

Model of GTPBP5 action during mtLSU biogenesis. (A) Interaction of E. coli ObgE with the 50S LSU (PDB 4CSU) (46). (B) Interaction of the human 39S mtLSU (PDB 6NU2) with mitochondrial ribosome recycling factor RRF1 (48). (C) Model depicting the mtLSU late-stages of assembly and the roles broadly performed by GTPBP5 and additional assembly factors in mtLSU maturation (see text for extended explanation). CP, central protuberance; L1 and L12 mark the position of the corresponding stalks. The surface representation of the structural profile of the human mitoribosome (PDB 3J9M) (1) and the structure of the ObgE homolog of GTPBP5 (PDB 4CSU) (46) were used. Figures in panels A and B were prepared using PYMOL software.