Figure 1.

miRNA-like small RNA fragments are produced from the U3 box C/D snoRNA. (A) Small RNA-seq was performed on total RNA extracted from HEK293 cells and the normalised numbers of reads of <40 nt in length mapping to different snoRNAs is shown as boxplots. Error bars represent the variation between three independent library preparations. (B) Hierarchical clustering was performed to analyse the distribution of reads mapping to selected snoRNA sequences in the small RNA-seq libraries. Nucleotide positions are indicated below the x-axis. (C) The distribution of sequence lengths of snoRNA-derived fragments and known miRNAs are shown as box blots. The black line represents the median and blue diamond shows the mean.