FIGURE 1.

RNA binding proteins that UV-crosslink poorly to RNA are widespread in RNA metabolism. (A) Venn diagram showing the overlap between RBPs defined based on UV crosslinking-dependent RNA interactome capture (RIC, from Hentze et al. 2018) and those defined by protein:protein interaction network of annotated RBPs (annotated and predicted RNA-associated proteins, from Brannan et al. 2016). (B) Venn diagram as in A showing overlap between integrated human RBPome defined based on UV-crosslinkability (ihRBPome, from Trendel et al. 2019) and annotated and predicted RNA-associated proteins (from Brannan et al. 2016) based on protein:protein interaction network of annotated RBPs. (C) Top sixteen gene ontology terms (biological process) enriched among RAFs. Legend on the right indicates the number of genes in each term. Some redundant GO terms were removed. (D) GO terms (cellular compartment) enriched among RAFs as in C. (E) GO terms (molecular function) enriched among RAFs as in C. (F) Major processes in the life-cycle of eukaryotic messenger RNAs (left) along with some examples of RAFs involved in each of the steps. Also shown are some examples of RAFs that are components of key membraneless RNP compartments or large RNP complexes (right).