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. 2020 Sep;26(9):1216–1233. doi: 10.1261/rna.074856.120

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© 2020 Patton et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article, published in RNA, is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 2. — Formaldehyde crosslinking stabilizes an RAF-EJC interaction. (A) Western blots showing proteins on the right in the total extract (TE) or FLAG immunoprecipitation (IP) fractions of HEK293 Flp-In cells expressing FLAG-MAGOH. The presence or absence of RNase A and the amount of NaCl present in the extracts during the IP are indicated above each lane. The dotted line indicates where gel images were spliced together. Data are representative of two biological replicates. (B) Western blots showing proteins (labeled on the right) in the insoluble pellet, soluble extract, and anti-FLAG immunoprecipitate fractions (indicated on the top) of HEK293 Flp-In cells. The lysis and IP conditions are also indicated on the top. Also indicated above each lane is the expression of FLAG-RNPS1 (+) or FLAG epitope only (−) in the cells, and the formaldehyde concentration used for in vivo crosslinking. Data are representative of three biological replicates.