FIGURE 2.

Expression of DeaD is autoregulated. (A) Expression of deaD mRNA in WT strains and in derivatives containing mutations in conserved motifs of DeaD. The position of the full-length deaD mRNA is indicated by an arrow. The normalized levels of deaD mRNA, along with their associated standard errors, are shown below. (B) Protein analysis. Total cellular proteins were separated by gel electrophoresis and the gels were dye-stained to visualize proteins. The band corresponding to DeaD is indicated by an arrow. The sizes of molecular weight markers run alongside the proteins is shown on the right. (C) β-galactosidase assays. Assays were performed in WT or ΔdeaD strains containing a multicopy deaD-lacZ fusion plasmid, in plasmid-containing strains that contained an additional ΔpcnB mutation, or in strains containing a single copy chromosomal deaD-lacZ fusion.