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A potential mechanism to explain the observed effects of transcription speed with the suboptical 5′ splice site GUACGU. Gray boxes, exons. (Upper) Base-pairing of a canonical 5′SS with U1 snRNA, and with U6 snRNA following Prp28 function. (Lower) Likely base-pairing of the non-canonical 5′SS, GUACGU, with U1 snRNA, and with U6 snRNA following Prp28 function. Vertical bars do not indicate the strength of base-pairing.
