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. 2020 Aug 17;14(8):e0008381. doi: 10.1371/journal.pntd.0008381

Fig 3. NGS-based microbial detection in blood.

Fig 3

(A) Normalized read counts mapping to the top 5 microbes detected by GOTTCHA at the species level are presented for patients adjudicated with Orientia tsutsugamushi infection. Sequencing was performed on blood collected at initial hospital entry. Patient samples are ordered by method of adjudication: IgG seroconversion, IgM seroconversion, 4-fold IgM titer increase, or IgM positive with no convalescent sample. (B) Mapping O. tsutsugamushi positive reads to a reference genome (NCBI Accession: NC_010793) demonstrates high coverage in two regions representing 16S (C) and 23S (D) ribosomal RNA. (E) Acute IgM, Blood Urea Nitrogen (bun; mg/dL), and Alanine Aminotransferase (ast; U/L) measurements for O. tsutsugamushi are presented for patients grouped by NGS-based detection where group 1, colored white, indicates no detection by NGS, and group 2, colored red, indicates O. tsutsugamushi was detected by all taxonomy tools.